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Products for labeling

 

DIG-High Prime DNA Labeling and Detection Starter Kit I
 高效DNA地高辛標記和檢測試劑盒I  
  現貨供應

Kits for labeling and detection

 

  

    比普通DIG隨機引物法標記效率高, 操作簡單, 可用于Southern、Northern和斑點雜交以及菌落和噬菌體篩選。一個Kit可用于12次標記反應和24次檢測反應。組成: 5×DIG-高效標記試劑; 未標記對照DNA, PBR328; DNA稀釋液; -DIG-堿性磷酸酶復合物; NBT/BCIP儲存液; 10×封閉液; DIG定量復試紙條; DIG對照試紙條, -20保存。

Application

DIG-High Prime labeled probes can be used in Southern-, northern- and dot-blot analysis, as well as colony and plaque hybridizations. This kit offers random-primed labeling of DNA templates with DIG-11-dUTP, alkali-labile, and color detection of the DIG-labeled hybrids. This kit was assembled with convenience in mind, offering a ready-made blocking solution, combined stock solution of NBT/ BCIP, and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow enzyme, nucleotides, primers, and reaction buffer, all in one convenient reagent.

Benefits

The High Prime method of labeling offers many benefits. Plasmid, cosmid, and lambda phage DNA have all been used as templates. We have achieved equal efficiency when labeling 10 ng to 3 µg of template DNA, which may be linear or supercoiled.

Product Description

Sensitivity and specificity: The standard assay uses 1 µg of linearized pBR328. It is usual to expect 0.8 µg labeled DNA after 1 hour of labeling, and 2.3 µg labeled DNA after a 20-hour reaction. In a dot-blot hybridization, 0.1 pg of homologous DNA is detectable after a 16-hour color development (probe concentrations at 20 ng/ml). A single-copy human gene (tPA) is detectable in a Southern blot, loading 1 µg of digested placenta DNA.

Contents

  1. DIG-High Prime, 5x conc., 50 µl
  2. DIG-labeled Control DNA, 20 µl, (5 µg/ml), pBR328 (linearized with Bam HI)
  3. DNA Dilution Buffer, 3 x 1 ml
  4. Anti-digoxigenin-AP Conjugate, 100 µl
  5. NBT/BCIP Stock Solution, conc., 6 x 1 ml
  6. Blocking Solution, 10x conc., 4 x 100 ml
  7. DIG Easy Hyb Granules, 4 x 100 ml
貨號 產品名稱 包裝 價格(元)
11745832910 DIG High Prime Lab/Detection K 1 kit (12 labeling reactions and 24 detection reactions) 點擊看報價

Kits for labeling

 

PCR product labeling

 

Mixes and reagents for labeling

 

Enzymes for labeling

 

Nucleotides for labeling

 

Columns to purify labeled nucleic acids

 

Products for detection

 

Kits for detection

 

Detection of PCR products in microplates

 

Anti-DIG conjugates for detection on membranes

 

Antibodies and conjugates for in situ hybridization

 

Chemiluminescent substrates for alkaline phosphatase

 

Chromogenic substrates for alkaline phosphatase

 

Substrates for peroxidase

 

Membranes and films for nonradioactive analysis

 

Products for hybridization

 

Other related products

 

Molecular weight markers, DIG-labeled

 

Nucleic acids and probes, DIG-labeled

 

Additional kits for nonradioactive assays

 

Additional reagents for in situ hybridization

 
 

 

 

 

 

 

 

 

 

 

 
 
 

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