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Products for labeling

 

DIG-High Prime DNA Labeling and Detection Starter Kit II
 高效DNA地高辛標記和檢測試劑盒II

Kits for labeling and detection

 

  

    比普通DIG隨機引物法標記效率高, 采用CSPD化學發光檢測靈敏度更高。一個Kit可用于12次標記反應和24次檢測反應。組成: 5×高效DIG標記復合物; 未標記對照DNA, PBR328; DNA稀釋液; 抗-DIG-堿性磷酸酶連接物; CSPD; 10×封閉液; DIG定量試紙條; DIG對照試紙條, NBT/BCIP儲存液; -20℃保存。

Application

DIG-High Prime labeled probes can be used in northern, Southern-, and dot-blot analysis, as well as colony and plaque hybridizations. This kit offers random-primed labeling of DNA templates with DIG-11-dUTP, alkali-labile, and chemiluminescent detection of the DIG-labeled hybrids. This kit was assembled with convenience in mind, offering ready-to-use CSPD supplied with a dripping device for easy application, ready-made blocking solution, and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow enzyme, nucleotides, primers, and reaction buffer, all in one convenient reagent.

Benefits

The High Prime method of labeling offers many benefits: in addition to the optimized labeling efficiency, plasmid, cosmid, and phage DNA, linearized or supercoiled can be used as templates, yielding the same labeling efficiency.

Product Description

Sensitivity and specificity: Standard assay uses 1 µg of linearized pBR328. It is usual to expect 0.8 µg labeled DNA after 1 h of labeling, and 2.3 µg labeled DNA after 20 h reaction. In a dot blot hybridization, 0.03 pg of homologous DNA is detectable with chemiluminescence (probe concentrations at 20 ng/ml). A single copy human gene (t-PA) is detectable in a Southern blot, loading 1 µg of digested placenta DNA.

Contents

  1. DIG-High Prime, 5x conc., 50 µl
  2. DIG-labeled Control DNA, 20 µl, (5 µg/ml) pBR328 (linearized with Bam HI)
  3. DNA Dilution Buffer, 3 x 1 ml
  4. Anti-digoxigenin-AP Conjugate, 50 µl
  5. CSPD, ready-to-use, 50 ml
  6. Blocking Solution, 10x conc., 4 x 100 ml
  7. DIG Easy Hyb Granules, 4 x 100 ml
貨號 產品名稱 包裝 價格(元)
11585614910 DIG High Prime Lab./Det.Kit II 1 kit (12 labeling reactions and 24 detection reactions) 點擊看報價

Kits for labeling

 

PCR product labeling

 

Mixes and reagents for labeling

 

Enzymes for labeling

 

Nucleotides for labeling

 

Columns to purify labeled nucleic acids

 

Products for detection

 

Kits for detection

 

Detection of PCR products in microplates

 

Anti-DIG conjugates for detection on membranes

 

Antibodies and conjugates for in situ hybridization

 

Chemiluminescent substrates for alkaline phosphatase

 

Chromogenic substrates for alkaline phosphatase

 

Substrates for peroxidase

 

Membranes and films for nonradioactive analysis

 

Products for hybridization

 

Other related products

 

Molecular weight markers, DIG-labeled

 

Nucleic acids and probes, DIG-labeled

 

Additional kits for nonradioactive assays

 

Additional reagents for in situ hybridization

 
 

 

 

 

 

 

 

 

 

 
 
 

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